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喜讯:恭贺南京农业大学王老师成功取奖学金

点击次数:395   发布时间:2021/5/11 10:38:15

 恒远生物 业致力于打造流的民族品牌,提供全面的、创新的、优质的、便捷的科研产品和服务。目,我们自主研发的重组蛋白、抗体、ELISA检测试剂盒得到广大科研用户的认可,并且多次发表于权威期刊,进步推动了民族品牌化的传播。

服务热线:13564766906
五月,大家的论文写的怎么样了?
先我们要恭贺下来自南京农业大学的王老师,2021年王老师在《International Journal of Nanomedicine》(纳米医学杂志)发表了SCI文献,发表的文献影响因子(IF)达到了5.15分!然后让我们恭喜王老师到了恒远生物的奖学金!
 
这篇论文写的非常棒,其中的很多实验方法和思路值得我们阅读,小编想要分享给大家,希望能够对做实验的小伙伴们有点点帮助,也希望大家积的参与我们的“发论文赢奖学金”活动,小编等着你们的投稿哦,下个奖学金的说不定就是你!
 
、恒远产品文献分享:
 
【文献标题】
Nanoparticles of Chitosan/Poly(D,L-Lactide-CoGlycolide) Enhanced the Immune Responses of Haemonchus contortus HCA59 Antigen in Model Mice
【作者单位】南京农业大学(Nanjing Agricultural University)
【文献中引用产品】
小鼠免疫球蛋白G1(IgG1)elisa试剂盒
小鼠IgG2a抗体亚型ELISA试剂盒
小鼠免疫球蛋白M(IgM)ELISA试剂盒
小鼠白介素4(IL-4)ELISA试剂盒
小鼠白介素12(IL-12)ELISA试剂盒
小鼠白介素17A(IL-17A)ELISA试剂盒
小鼠γ干扰素(IFN-γ)ELISA试剂盒
小鼠转化生长因子β(TGF-β) ELISA试剂盒
【关键词】
【DOI】https://doi.org/10.2147/IJN.S301851 
【影响因子(IF)】5.15
【出版期刊】《International Journal of Nanomedicine》
 
二、产品原文引用
 
Cytokine Assays
The levels of IL-4, IL-12, IL-17, IFN-γ, and TGF-β in sera from the ten groups were investigated with commercial ELISA kits (HengYuan, Shanghai, China) according to the manufacturer’s guidelines.
 
 
Characterization of rHCA59-CS NPs and rHCA59-PLGA NPs

After conjugation, PLGA and Chitosan nanoparticles were precipitated and the free amounts of rHCA59 protein were determined by a BCA protein assay kit. It was found that 89% and 71% of rHCA59 were conjugated to PLGA and Chitosan, respectively. In other words, a total of 1.78 mg of protein bound to PLGA, and 1.42 mg of protein bound to Chitosan.A scanning electron microscopy was used to identify the size and morphology of PLGA and Chitosan nanoparticles. The SEM results showed that both PLGA and Chitosan nanoparticles’ appearances were smooth. The size of rHCA59-PLGA NPs was from 180 nm to 402 nm (Figure 1A) and the size of rHCA59-CS NPs was from 200 nm to 500 nm (Figure 1B).


 
 
Figure 3 Effects of different antigen delivery systems on the expressions of multiple cytokines. Sera were collected and detected on day 14 by ELISA. 

Notes: (1): PBS group (blank control). (2): pET-32a protein group. (3): PBS-PLGA group. (4): PBS-CS group. (5): PBS-CFA group. (6): rHCA59 group. (7): rHCA59-PLGA group. (8): rHCA59-CS group. (9): rHCA59-CFA group. (10): rHCA59-CS-PLGA group. (A) IL-12. (B) IL-17. (C) IFN-γ. (D) IL-4. (E) TGF-β. Data are representative of triplicate independent experiments (*p < 0.05, **p < 0.01, and ***p < 0.001). Abbreviations: PLGA, poly-D,L-lactide-co-glycolide; CS, Chitosan; CFA, complete Freund’s adjuvant; IL, interleukin; ELISA, enzyme linked immunosorbent assay


 
 
Figure 5 Effects of different antigen delivery systems on T lymphocyte subsets.

Notes: rHCA59 loaded nanoparticles could affect the proportions of CD4+ T cells and CD8+ T cells in immunized mice. The percentages of CD4+ T cells (A1–A10) and CD8+ T cells (B1–B10) in ten groups were investigated by flow cytometry on day 14 after immunization. (C and D) Different treatments affected the proportions of CD4+ T cells and CD8+ T cells. (A1 and B1) PBS group (blank control). (A2 and B2) PBS-CFA group. (A3 and B3) PBS-PLGA group. (A4 and B4) PBS-CS group. (A5 and B5) pET-32a protein group. (A6 and B6) rHCA59 group. (A7 and B7) rHCA59-CFA group. (A8 and B8) rHCA59-CS group. (A9 and B9) rHCA59-PLGA group. (A10 and B10) rHCA59-CS-PLGA group. The results shown here are from three independent experiments (*p < 0.05, **p < 0.01, and ***p < 0.001). Abbreviations: CD4, cluster of differentiation 4; CD8, cluster of differentiation 8; T cells, thymus cells; PLGA, poly-D,L-lactide-co-glycolide; CS, Chitosan; CFA, complete Freund’s adjuvant.

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