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点击次数:3612 发布时间:2022/5/12 10:12:44
研究意义:
细胞中,细胞色素P450主要分布在内质网和线粒体内膜上,作为一种末端加氧酶,参与了生物体内的甾醇类激素合成等过程。近年来,对细胞色素P450的结构、功能特别是对其在药物代谢中的作用的研究有了较大的进展。研究表明细胞色素P450是药物代谢过程中的关键酶,而且对细胞因子和体温调节都有重要影响。
相关文献:
【文献标题】Enantioselective toxicity and oxidative stress effects of acetochlor on earthworms (Eisenia fetida) by mediating the signaling pathway
【作者】Yalei Liu, Kuan Fang, Xiaolian Zhang,et.al
【作者单位】中国农业科学院烟草研究所(Tobacco Research Institute of Chinese Academy of Agricultural Sciences)
【文献中引用产品】
蚯蚓8-羟基脱氧鸟苷(8-OHdG)ELISA试剂盒
蚯蚓细胞色素P450(CYP450)ELISA试剂盒
蚯蚓羟基自由基(OH)ELISA检测试剂盒
【关键词】Acetochlor,Enantiomer,Earthworm,Toxicity,Transcriptomic
【DOI】doi.org/10.1016/j.scitotenv.2020.142630
【影响因子(IF)】8.6
【出版期刊】《Science of the Total Environment》
【产品原文引用】
The OH content, 8-OhdG content, and CYP450 content were determined according to the method of Zhang et al. (2020) using a kit obtained from the ShangHai HengYuan Biological Technology Co., Ltd. (Shanghai, China). The OH content, 8-OHdG content, and activity of P450 in earthworms were detected by the double antibody sandwich method. The OH in earthworms combined with the antibodies of OH marked by HRP (Horseradish Peroxidase). Then, 3,3′,5,5′-tetramethylbenzidine (TMB) was added to produce a chromogenic reaction with the compound of antibody-antigen-antibody labeled by the enzyme. The absorbance of the reaction solution was recorded at 450 nm. The detection principle and method of 8-OhdG and CYP450 are the same as those of OH.
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原创作者:上海恒远生物科技有限公司