Sigma (St Louis, MO, USA) provided the DHEA, dimethyl sulfoxide (DMSO) ａnd LPS (Escherichia coli 055:B5). The Opti-MEM medium ａnd fetal bovine serum (FBS) were obtained from Gibco (Erie, NY, USA). The dulbecco＇s modified Eagle＇s medium (DMEM) ａnd trypsin-EDTA were all obtained from Biological Industries (Kibbutz Beit-Haemek, Israel). Corning (NY, USA) provided the cell culture dishes.
TRIZOL Reagent Kits, SYBR Green Master Mix ａnd reverse transcriptase kit were all purchased from Vazyme Biotech Co., Ltd. (Nanjing, China). Jiancheng Bioengineering Institute of Nanjing (Nanjing, China) provided the Nitric Oxide (NO), malondialdehyde (MDA) ａnd total superoxide dismutase (SOD) detection kits. Shanghai hengyuan biological technology Co., Ltd. (Shanghai, China) provided the
TNF-α, IL-1β ａnd Estradiol (E2) ELISA kits. Beyotime Biotechnology Institute (Shanghai, China) provided the glutathione peroxidase, nitric Oxide (NO), protein contents assay kits ａnd N-Acetyl-L-cysteine (NAC). MCE (St. Louis, MO, USA) provided the inhibitors including G1, G15, SB203580, U0126, BAY11-7082, GKT137831, TAK-242, 3-MA, Fulvestrant, Flutamide, ML385 ａnd RTA-408.
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