【文献标题】A safe ａnd efficient bioactive citrate-lysine/miRNA33 agonist nanosystem for high fat diet-induced obesity therapy【作者】Long Zhang, Min Wang, Min Wang,et.al
【作者单位】西安交通大学(Xi’an Jiaotong University)
【文献中引用产品】
大鼠低密度脂蛋白（LDL)ELISA试剂盒
【关键词】Bioactive biomaterials ,Polycitrate ,miRNA delivery ,Obesity therapy
【DOI】https://doi.org/10.1016/j.cej.2020.127304
【影响因子(IF)】15.2
【出版期刊】《Chemical Engineering Journal》
【产品原文引用】
On day 21, the enzyme linked immunosorbent assay (ELISA) analysis was carried out on the serum sample. High density lipoprotein (HDL,Hengyuan), low density lipoprotein (LDL, Hengyuan), leptin (RD) ａnd insulin (Alpco) were measured according to the manufacturers’ instruction. Three repeats were used ａnd the microplate reader was used to test the signal (Thermo Fisher). Total RNA was isolated ａnd purified using RNAiso Plus (TaKaRa) from abdominal fat (WAT, white adipose tissue) ａnd liver. And cDNA was synthesized using the Transcriptor First Strand cDNA Synthesis Kit (Roche) in accordance with the manufacturer’s instructions. Primers information was shown in Table S1.
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